Identification of a novel lncRNA GABPB1-IT1 that is downregulated and predicts a poor prognosis in non-small cell lung cancer

Abstract

Lung cancer is the leading cause of cancer-associated mortality worldwide. Previous studies have demonstrated that long non-coding RNAs (lncRNAs) serve important roles in diverse biological processes. However, the molecular function and prognostic value of the majority of lncRNAs in non-small cell lung cancer (NSCLC) remain unknown. The present study investigated the expression of the lncRNA GABPB1 intronic transcript (GABPB1-IT1) in NSCLC tissues using publicly available databases. Subsequently, protein-protein interaction (PPI) and co-expression networks were constructed to identify key targets of lncRNA GABPB1-IT1. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to investigate the potential roles of lncRNA GABPB1-IT1. The current study identified that the expression of GABPB1-IT1 was significantly downregulated in NSCLC samples compared with normal samples. Furthermore, the expression levels of GABPB1-IT1 were lower in high grade NSCLC samples compared with low grade NSCLC samples. Additionally, overexpression of GABPB1-IT1 in cancer samples was associated with improved survival of patients with NSCLC. GABPB1-IT1 was revealed to be involved in the regulation of cell cycle-associated biological processes, including sister chromatid cohesion, mitotic nuclear division, DNA replication, chromosome segregation, G1/S transition of mitotic cell cycle, mitotic cytokinesis and cell division. Finally, a GABPB1-IT1-associated protein-protein interaction network was constructed for NSCLC. To the best of our knowledge, the present study was the first to demonstrate that GABPB1-IT1 is associated with the prognosis of NSCLC. The current study provides useful information to assist with the investigation of potential candidate biomarkers for diagnosis, prognosis and drug targets for NSCLC.