Immobilization and Biochemical Properties of Purified Xylanase from Bacillus amyloliquefaciens SK-3 and Its Application in Kraft Pulp Biobleaching

Abstract

Abstract In the present study, we studied the production and immobilization xylanase from Bacillus amyloliquefaciens strain SK-3. Isolate produced highest xylanase activity (56.2±1.6 IU/mol) at pH= 8 and 40 C after 48 h incubation in presence of 1% wheat bran. Immobilization studies of purified xylanase showed that 3.0% sodium alginate and 0.2 M calcium chloride was found to be optimum. Characterization of immobilized beads by SEM and FTIR showed significant changes on the surface morphology and structure.