Biotyping of Campylobacter strains isolated in Lagos, Nigeria using the modified preston biotype

Abstract

Fifty-eight Campylobacter strains were isolated from children with diarrhoea at various health centres in Lagos and from healthy chicken. Twenty - nine strains of Campylobacter were isolated from humans, while the same number were isolated from chicken. The strains were biotyped using the modified Preston biotype scheme. The Preston biotyping results have been compared with the results of Penner serotyping. Out of fifty-eight strains studied, the technique identified ten strains (17%) as C. coli, three (5%) as C. lari and fourty-five (78%) as C. jejuni, by the coding system. This technique identified twenty-eight Campylobacter species. This method highlights the usefulness of this technique in the biotyping of local strains, however, when the two schemes are used in combination they give excellent typing results suitable for epidemiological purposes.