Mechanistic investigations of unsaturated glucuronyl hydrolase from clostridium perfringens

Abstract

Background: Unsaturated glucuronyl hydrolases (UGL) from GH88 are bacterial enzymes involved in the degradation of glycosaminoglycans and are virulence factors. Results: Mechanistic data inconsistent with the currently accepted mechanism of UGL are presented. Conclusion: A revised mechanism is proposed to explain all mechanistic data published to date. Significance: Understanding of the mechanism of UGL may allow design of bacteriostatic agents. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.