Effect of Indole 3-Acetic Acid (IAA) and 6-Benzyl Amino Purine (BAP) on Nannochloropsis sp. culture growth

Abstract

One of the factors influencing the growth of Nannochloropsis sp. is the composition of culture media. The addition of growth regulators in the form of auxins and cytokines in culture media can increase the growth of microalgae. This study aims to examine the effect of IAA (auxin) and BAP (cytokinin) with various concentrations on biomass, chlorophyll content and carbohydrate content of Nannochloropsis sp. culture. Nannochloropsis sp. culture was treated with IAA and BAP in concentration variations each consisting of 0, 0.1, 1, 10 ppm with 3 replications. Data were analyzed with two way ANOVA at 95% confidence level and Tukey follow-up test. The results showed that the combination treatment of IAA and BAP did not affect the chlorophyll-a content of Nannochloropsis sp. culture, but it affected the biomass with the highest P16 (I10B10) of 3.65 g/L and carbohydrate content with the highest content in P4 (I10B0) of 0.30 mg/L. The highest chlorophyll-a content was found in P15(I1B10) of 5.574 mg/L, increased by 6 % compared to controls. Whereas the lowest chlorophyll-a content was found in P12(I10B1) of 1.563 mg/L, decreased by 70 % compared to the control.