Adenine, an inhibitor of platelet aggregation, from the leaves of Cassia alata

Abstract

Adenine was isolated as a platelet aggregating inhibitor from the leaves of Cassia alata by HPLC using a triacontylsilyl silica (C30) column. The inhibitory effects of adenine and adenosine (positive control) on the platelet aggregation induced by collagen or adenosine 5′-diphosphate (ADP) as an aggregating agent was evaluated with a platelet aggregometer using a laser-scattering method. As a result, the inhibitory effect of adenine was observed in the platelet aggregation induced by collagen (1.0 μg/ml as the final concentration), but little inhibitory effect was noted in the aggregation induced by ADP (5.0 μM as the final concentration), whereas adenosine exhibited potent inhibitory effects on platelet aggregation induced both by collagen and ADP under the same experimental conditions. © 2003 Pharmaceutical Society of Japan.