Highly sensitive and non-disruptive detection of residual undifferentiated cells by measuring miRNAs in culture supernatant

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Abstract

The clinical usage of induced pluripotent stem cell (iPSC)-derived regenerative medicine products is limited by the possibility of residual undifferentiated cells forming tumours after transplantation. Most of the existing quality control tests involve crushing of cells. As a result, the cells to be transplanted cannot be directly tested, thereby increasing the cost of transplantation. Therefore, we tested a highly sensitive and non-disruptive quality-testing method that involves measuring microRNAs (miRNAs) in culture supernatants released by cells. By measuring miR-302b in the culture supernatant, residual iPSCs were detected with higher sensitivity than by measuring LIN28 (Lin-28 Homolog A) in the cells. To use this method, we also monitored the progression of differentiation. Our novel highly sensitive and non-disruptive method for detecting residual undifferentiated cells will contribute to reducing the manufacturing cost of iPSC-derived products and improving the safety of transplantation.

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Masumoto, K., Aihara, Y., Miyagawa Kuroishi, M., Maeda, N., Sakai, Y., Oka, Y., … Yanagida, M. (2022). Highly sensitive and non-disruptive detection of residual undifferentiated cells by measuring miRNAs in culture supernatant. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-14273-z

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