Structure of the constant and 3′ untranslated regions of the murine balb/c γ2a heavy chain messenger RNA

Abstract

The complete sequence for the constant and 3′ untranslated regions of a mouse γ2a immunoglobulin heavy chain mRNA is reported. The sequence is 1093 nucleotides long coding for the CH1 (amino-acids 118-214), the Hinge (215-230), the CH2 (231-340) and the CH3 (341-447). The 3′ untranslated region is 103 nucleotides long preceding the poly(A). The nucleotide sequence predicts as in the case for γ1 and γ2b heavy chains an additional lysine residue before the termination codon. This sequence has been compared to the corresponding sequences of γ1 and γ2b heavy chain mRNAs. These sequences are respectively 75% and 84% homologous. The CH2 domains of γ2a and γ2b are 95% homologous at the nucleotide level. The cross-over point of a γ2a γ2b heavy chain variant is located in a segment of 73 perfectly matching nucleotides. The 3′ non coding regions of γ2a and γ2b are 89% homologous. © 1980 IRL Press Limited.