Clone and Sequence Analysis of Cu/Zn-SOD Gene from Saccharum arundinaceum Retz.

Abstract

Saccharum arundinaceum has strong drought tolerance, the abundant stress tolerance gene resource could be used in sugarcane breeding programs. Superoxide dismutase (SOD) is a crucial enzyme in reactive oxygen species (ROS) action, which are likely to function as components of a stress-signaling pathway. To investigate the gene of SOD, by using a homologous cloning strategy, a Cu/Zn-SOD cDNA sequence(NCBI accession number XM- 002445626.1) in Sorghum was as probe to retrieve, aligned and spliced with Saccharum EST database, a full length of Cu/Zn-SOD gene named SaSOD-1b (NCBI accession number: KJ 002570) from Saccharum arundinaceum was cloned. The cloned gene sequence was 692bp, including 136bp 5 untranslated regions (UTR), 162bp ORF and 135bp 3 UTR, encoded 206 amino acids. By bioinformatics analysis, the cloned genic protein molecular weight was 20.78kDa, and pI was 5.32, the amino acid sequence owned the Cu/Zn-SOD conserved domain, and it had high similarity with other plants Cu/Zn-SOD. The phylogenetic tree analysis indicated that the cloned gene was most closely relative with Sorghum Cu/Zn-SOD gene, this study could laid a foundation for further understanding the relation between SOD and Saccharum arundinaceum drought resistance.