Hmi1p from Saccharomyces cerevisiae mitochondria is a structure-specific DNA helicase

Abstract

Hmi1p is a Saccharomyces cerevisiae mitochondrial DNA helicase that is essential for the maintenance of functional mitochondrial DNA. Hmi1p belongs to the super-family 1 of helicases and is a close homologue of bacterial PcrA and Rep helicases. We have overexpressed and purified recombinant Hmi1p from Escherichia coli and describe here the biochemical characteristics of its DNA helicase activities. Among nucleotide cofactors, the DNA unwinding by Hmi1p was found to occur efficiently only in the presence of ATP and dATP. Hmi1p could unwind only the DNA substrates with a 3′-single-stranded overhang. The length of the 3′-overhang needed for efficient targeting of the helicase to the substrate depended on the substrate structure. For substrates consisting of duplex DNA with a 3′-single-stranded DNA overhang, at least a 19-nt 3′-overhang was needed. In the case of forked substrates with both 3′- and 5′-overhangs, a 9-nt 3′-overhang was sufficient provided that the 5′-overhang was also 9 nt in length. In flap-structured substrates mimicking the chain displacement structures in DNA recombination process, only a 5-nt 3′-single-stranded DNA tail was required for efficient unwinding by Hmi1p. These data indicate that Hmi1p may be targeted to a specific 3′-flap structure, suggesting its possible role in DNA recombination. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.