Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

81Citations
Citations of this article
164Readers
Mendeley users who have this article in their library.
Get full text
This PDF is freely available from an open access repository. It may not have been peer-reviewed.

Abstract

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called “CRISPR-plus” (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

Cite

CITATION STYLE

APA

Jain, P. K., Ramanan, V., Schepers, A. G., Dalvie, N. S., Panda, A., Fleming, H. E., & Bhatia, S. N. (2016). Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors. Angewandte Chemie - International Edition, 55(40), 12440–12444. https://doi.org/10.1002/anie.201606123

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free