Compatibility of translational enhancers with various plant species

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Abstract

Translational enhancers are effective tools to increase the expression level of transgenes in plant cells, and some candidate elements are in use today. However, knowledge about suitable elements for a given plant species are limited. We aim here to make a catalogue of translational enhancers, and evaluated the effectiveness of 5′-UTRs of Nicotiana tabacum alcohol dehysrogenase gene (NtADH 5′-UTR), Arabidopsis thaliana ADH gene (AtADH 5′-UTR), Oryza sativa ADH gene (OsADH 5′-UTR), Ω derived from tobacco mosaic virus, and tobacco etch virus leader (TEVL) in various plant species. We found that the OsADH 5′-UTR is functional in all plant species tested, and that Ω and TEVL are effective in eudicots but not in monocots. From these results, we speculate that the degree of translational enhancement of any element in a given plant species is closely correlated with the phylogenic position of the species.

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Matsui, T., Sawada, K., Takita, E., & Kato, K. (2015). Compatibility of translational enhancers with various plant species. Plant Biotechnology, 32(4), 309–316. https://doi.org/10.5511/plantbiotechnology.15.1103a

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