The human OCTN1 (SLC22A4) reconstituted in liposomes catalyzes acetylcholine transport which is defective in the mutant L503F associated to the Crohn's disease

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Abstract

The organic cation transporter (OCTN1) plays key roles in transport of selected organic cations, but understanding of its biological functions remains limited by restricted knowledge of its substrate targets. Here we show capacity of human OCTN1-reconstituted proteoliposomes to mediate uptake and efflux of [ 3H]acetylcholine, the Km of transport being 1.0 mM with V max of 160 nmol·mg - 1 protein·min - 1. OCTN1-mediated transport of this neurotransmitter was time-dependent and was stimulated by intraliposomal ATP. The transporter operates as uniporter but translocates acetylcholine in both directions. [ 3H]acetylcholine uptake was competitively inhibited by tetraethylammonium, γ-butyrobetaine and acetylcarnitine, and was also inhibited by various polyamines. Decreasing intraliposomal ATP concentrations increased OCTN Km for acetylcholine, but V max was unaffected. Evaluation of the acetylcholine transporter properties of a variant form of OCTN1, the Crohn's disease-associated 503F variant, revealed time course, Km and V max for acetylcholine uptake to be comparable to that of wild-type OCTN1. Km for acetylcholine efflux was also comparable for both OCTN1 species, but V max of OCTN1 503F-mediated acetylcholine efflux (1.9 nmol·mg - 1 protein·min - 1) was significantly lower than that of wild-type OCTN1 (14 nmol·mg - 1 protein·min - 1). These data identify a new transport role for OCTN1 and raise the possibility that its involvement in the non-neuronal acetylcholine system may be relevant to the pathogenesis of Crohn's disease. © 2011 Elsevier B.V. All rights reserved.

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Pochini, L., Scalise, M., Galluccio, M., Pani, G., Siminovitch, K. A., & Indiveri, C. (2012). The human OCTN1 (SLC22A4) reconstituted in liposomes catalyzes acetylcholine transport which is defective in the mutant L503F associated to the Crohn’s disease. Biochimica et Biophysica Acta - Biomembranes, 1818(3), 559–565. https://doi.org/10.1016/j.bbamem.2011.12.014

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