Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Abstract

Cerium oxide nanoparticles (NanoCeO2) and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of NanoCeO2 increases effective electro-active surface area of CH-NanoCeO2 / indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. BSA/r-IgGs/ CH-NanoCeO2 /ITO immunoelectrode exhibits improved linearity (0.25-6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity (18 μA/ng dl-1 cm-2), and regression coefficient (r2 ∼0.997). © 2009 American Institute of Physics.