Genetic map construction and fiber quality QTL mapping using the cottonSNP80K array in upland cotton

Abstract

Cotton fiber quality traits are controlled by multiple quantitative trait loci (QTL), and the improvement of these traits requires extensive germplasm. Herein, an Upland cotton cultivar from America, Acala Maxxa, was crossed with a local high fiber quality cultivar, Yumian 1, and 180 recombinant inbred lines (RILs) were obtained. In order to dissect the genetic basis of fiber quality differences between these parents, a geneticmap containing 12116 SNP markers was constructed using the CottonSNP80K assay, which covered 3741.81 cMwith an average distance of 0.31 cMbetweenmarkers. Based on the genetic map and growouts in three environments, we detected a total of 104 QTL controlling fiber quality traits. Among these QTL, 25 were detected in all three environments and 35 in two environments. Meanwhile, 19 QTL clusters were also identified, and nine contained at least one stable QTL (detected in three environments for a given trait). These stable QTL or QTL clusters are priorities for fine mapping, identifying candidate genes, elaboratingmolecularmechanisms of fiber development, and application in cotton breeding programs by marker-assisted selection (MAS).