Enzyme kinetics: Partial and complete non-competitive inhibition

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Abstract

A novel graphical method for analysing the rates of enzyme reactions to obtain kinetic parameters K(i), K(i) and β (degree of partiality) and to identify the types of inhibition and the enzyme mechanisms is described. The method consists of plotting experimental data as v/(V(o)-v) versus 1/(I) at different substrate concentrations. I is the inhibitor concentration, V(o) and v are the rates of enzyme reaction attained by the system in the presence of a fixed amount of substrate and in the absence and presence of inhibitor respectively. Complete inhibition gives a single straight line that passes through the origin while partial inhibition gives a straight line that intercepts the 1/(I) axis at a point away from the origin.

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Whiteley, C. G. (1999). Enzyme kinetics: Partial and complete non-competitive inhibition. Biochemical Education, 27(1), 15–18. https://doi.org/10.1016/S0307-4412(98)00265-9

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