Sperm morphological assessments of Bali boar semen collected from three area in Bali Island, Indonesia

Abstract

Bali pigs is one of the original germplasm of Bali Island. This study assessed the sperm morphology of Bali boars collected from three areas in Bali Island. Sperm morphology is known to be closely related to boar fertility and sterility. Total of 15 Bali boars were used in this study and sperm observations for each ejaculation. One drop of semen was placed on two or three glass slides at each ejaculation for examination. Sperm staining used was the carbolfuchsin-eosin (Williams staining method). Observation of sperm abnormality was carried out on 200-250 spermatozoa in each semen sample. The results showed that the sperm morphology of Bali boars in the three districts was not significantly different (P>0.05). The types of abnormality found in Bali boar semen were bent tail and coiled tail. The percentage of sperm abnormality in Bali boars was less than 20%. Keywords: