Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library

Abstract

Mammalian cell nuclei contain multiple granular structures, which are termed nuclear bodies. These structures are involved in various molecular events in the nucleus; they provide platforms for biogenesis of macromolecular complexes that are essential for gene expression, such as the ribosome and spliceosome; they act as reservoirs of various regulatory factors; and they are involved in the regulation of specifi c gene loci. Nuclear bodies are usually visualized by immunostaining for specifi c marker proteins. Although each type of nuclear body contains a distinct set of proteins, the protein components of most types of nuclear bodies remain to be identifi ed. This chapter introduces a new approach to identify the protein components of specifi c types of nuclear bodies.