Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product

Abstract

We attempted to clone an inosine kinase gene of Escherichia coli. A mutant strain which grows slowly with inosine as the sole purine source was used as a host for cloning. A cloned 2.8-kbp DNA fragment can accelerate the growth of the mutant with inosine. The fragment was sequenced, and one protein of 434 amino acids long was found. This protein was overexpressed. The overexpressed protein was purified and characterized. The enzyme had both inosine and guanosine kinase activity. The V(max)s for guanosine and inosine were 2.9 and 4.9 μmol/min/mg of protein, respectively. The K(m)s for guanosine and inosine were 6.1 μM and 2.1 mM, respectively. This enzyme accepted ATP and dATP as a phosphate donor but not p-nitrophenyl phosphate. These results show clearly that this enzyme is not a phosphotransferase but a guanosine kinase having low activity with inosine. The sequence of the gene we have cloned is almost identical to that of the gsk gene (K. W. Harlow, P. Nygaard, and B. Hove-Jensen, J. Bacteriol. 177:2236-2240, 1995).