Label-free electrochemical detection of an entamoeba histolytica antigen using cell-free yeast-scFv probes

Abstract

Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents. © 2013 The Royal Society of Chemistry.