Antioxidant and Anticoagulant Activity of Microbial Nano Cellulose-ZnO-Ag Composite Components

Abstract

Background and Objectives: Bacterial cellulose (BC) is a microbial extracellular biopolymer formed by microbial strains like Gluconacetobacter xylinus. The objective of this study was to determine the antioxidant and anticoagulant of a microbial nano cellulose-ZnO-Ag (CNCs) composite and its components separately. Materials andMethods: Three components were used for nano cellulose-ZnO-Ag composite synthesis, Ag-nanoparticles, ZnO-nanoparticles and BC. The DPPH method was used to calculate the scavenging of free radical behaviour of four different composite samples. Results: Results of silver nanoparticles were found to have the highest antioxidant activity with IC50 65 µg mL-1, followed by CNCs-ZnO-Ag composite (IC50 88.98 µg mL-1) but ZnONPs IC50 was 263 µg mL-1 and BC (IC50 955 µg mL-1). The CNCs-ZnO-Ag composite, BC and AgNPs at 25 µg mL-1 had clotting times that were nearly identical to the control. The APTT increased to 56 Sec at 75 µg mL-1 of CNCs-ZnO-Ag composite related to control that recorded 33 Sec. Conclusion: Bacterial cellulose acquired new activity in nano form and also when conjugated with nanoparticles. The CNCs-ZnO-Ag composite is ready for pharmaceutical application as an antioxidant and anticoagulant after in vivo study.