Lipocortin 1 binding sites on human T-cells: The population of cells with the binding sites is larger in CD8+ T-lymphocytes than in CD4+ T-lymphocytes

Abstract

Lipocortin 1 (LC1) is believed to be a mediator of glucocorticoids in displaying anti-inflammatory and immune suppressive responses. The existence of specific LC1 binding sites (putative receptor) on monocytes and neutrophils has been reported. We have studied the distribution of LC1 binding sites in human peripheral blood leukocytes by flow cytometry. The population of cells with LC1 binding sites was much larger in monocytes than in lymphocytes in both rheumatoid arthritis patients (93.1 ± 2.3% vs 8.8 ± 1.0%) and healthy volunteers (97.0 ± 0.9% vs 9.9 ± 1.5%). The LC1 binding cell population was larger in CD8+ T-lymphocytes than in CD4+ T-lymphocytes in both healthy volunteers (26.4 ± 4.5% vs 10.6 ± 2.0%) and rheumatoid arthritis patients (28.8 ± 4.7% vs 8.7 ± 2.1%). No difference in LC1 binding cell populations was found between rheumatoid arthritis patients and healthy controls.