Neurons and their function of conveying information across a chemical synapse are highly regulated systems. Impacts on their functional viability can occur independently from changes in morphology. Here we describe a method to assess the size of synaptic vesicle pools using live cell fluorescence imaging and a genetically encoded probe (pHluorin). Assessing functional parameters such as the size of synaptic vesicle pools can be a valuable addition to common assays of neuronal cell viability as they demonstrate that key cellular functions are intact.
CITATION STYLE
Wrosch, J. K., & Groemer, T. W. (2017). Functional viability: Measurement of synaptic vesicle pool sizes. In Methods in Molecular Biology (Vol. 1601, pp. 195–204). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6960-9_15
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