Phylogenetic analysis of selected olive genotypes by issr markers

Abstract

The genetic relationships among six different olive (Olea europaea L.) genotypes were determined through ten ISSR primers by obtaining informative profiles using five of them. The studied cultivars ‘Chondrolia Chalkidikis’, ‘Karidolia,’ ‘Prodromos,’ ‘Kalamon,’ ‘Koroneiki,’ and ‘Leucocarpa’ are widespread and grown in Chalkidiki and Northern Greece. By analysis of the amplification, a total of 34 bands were observed, and it was noticed that 26 of them were polymorphic, showing the high efficiency and reliability of the markers used. The highest number of ISSR bands detected for primer E4 was 10 (8 polymorphic). The calculated percentage of polymorphism was 76.5. A cluster analysis was performed based on the molecular characterization results, and a dendrogram was constructed using average linkage. The current genotyping presents distinctive profiles useful for proving olive planting material's cultivar and clonal identity.