In vitro culture of Dendrobium lineale Rolfe orchid for plant breeding and propagation

Abstract

D.lineale is included in the CITES appendix II due to D. lineale has great potential as a cut flower and the parent of hybrid orchids. In vitro culture can be used for mass propagation of orchids for commercial breeding as well as conservation. Basic culture medium with the addition of organic substances can increase the growth of plantlets. The objective of this research was to determine the best condition for in vitro culture of D.lineale orchids for producing a mass number of plantlets. Methods used include planting orchid seedlings in various variations of culture media, plantlet subcultures, observation of the anatomy of leaves and roots, as well as the measurement of chlorophyll content. This research uses 'pisang raja' as organic material in the culture medium. The highest chlorophyll content in the leaves was 1.58 mg/g, while the highest chlorophyll content in the roots was 0.34 mg/g. Both were found in medium NP+100 g/L bananas in light conditions (1317 lux). The anatomical structure of the leaves shows that the mesophyll is thicker in plants placed under lighting 1317 lux because it shows good effectiveness in photosynthesis. The anatomy of the orchid roots showed differences in diameter in each treatment because the addition of bananas to the medium stimulated rapid and abundant root formation, but the size was smaller. Based on these results, it can be concluded that the addition of 100 gr/L bananas as organic substances in in vitro medium culture increases the growth rate of D. lineale orchids, which will be beneficial for conservation efforts.