Improved in vitro Cultivation of Babesia caballi

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Abstract

Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O2, 5% CO2 and 93% N2). All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO2 in air, with parasitemia ranging from 8-10%.

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Avarzed, A., Igarashi, I., Kanemaru, T., Hirumi, K., Omata, Y., Saito, A., … Suzuki, N. (1997). Improved in vitro Cultivation of Babesia caballi. Journal of Veterinary Medical Science, 59(6), 479–481. https://doi.org/10.1292/jvms.59.479

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