Movement of the 3'-end of 16 S RNA towards S21 during activation of 30 S ribosomal subunits

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Abstract

Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol of ribosomal protein S21. The conformational change causes an apparent decrease in distance between the probes from 59 to 52 Å as determined by non-radiative energy transfer. © 1984.

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Odom, O. W., Stöffler, G., & Hardesty, B. (1984). Movement of the 3’-end of 16 S RNA towards S21 during activation of 30 S ribosomal subunits. FEBS Letters, 173(1), 155–158. https://doi.org/10.1016/0014-5793(84)81037-6

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