Isolation of Escherichia Coli from Diarrhea and Test Their Pathogensity and Susceptibility Pattern for Antibiotic

Abstract

This study was design to isolation and biochemical identification of Escherichia coli from diarrhea in children,\rtest susceptibility pattern for antibiotic and pathogenesis of Escherichia coli. For these reason 150 samples of stool\rgathered from patients children in different region of Dhi-Qar city in Iraq for the period Jaunuary 2015 to June of the\rsame year. Results of the bacterial growth showed that 111 samples were positive bacterial growth with percentage (74%)\r, isolate were microscopically and biochemically examined and diagnosed by Api 20E kit, The results showed 84 isolate\rbelong to Escherichia coli with percentage 75.6% .\rThe sensitivity of isolates were examined for antibiotics, the isolates showed a height resistance for Ampicillin,\rwhile the others showed highest sensitivity for Co – trimoxazol ,Erythromycin , Chloramphenicol.\rThe study showed the ability of Escherichia coli to elicit an inflammatory response in mouse intestine after\rexperimental infection that induced by orally dosing with Escherichia coli.