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Array-based yeast two-hybrid screens: A practical guide

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Abstract

Yeast two-hybrid screens are carried out as random library screens or matrix-based screens. The latter have the advantage of being better controlled and thus typically give clearer results. In this chapter, we provide detailed protocols for matrix-based Y2H screens and give some helpful instructions how to plan a large-scale interaction screen. We also discuss strategies to identify or avoid false negatives and false positives. © 2012 Springer Science+Business Media, LLC.

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Häuser, R., Stellberger, T., Rajagopala, S. V., & Uetz, P. (2012). Array-based yeast two-hybrid screens: A practical guide. Methods in Molecular Biology, 812, 21–38. https://doi.org/10.1007/978-1-61779-455-1_2

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