Antibacterial potential of butterfly Pea (Clitoria ternatea) towards Aggregatibacter actinomycetemcomitans in vitr

Abstract

Background: Periodontal disease in Indonesia have prevalence of 96.58%, One of the examples of periodontal disease is aggressive periodontitis caused by Aggregatibacter actinomycetemcomitans (Aa). Many researches have been done to study compounds that has antibacterial potential against Aa bacteria. Butterfly Pea is a plant abundant in Indonesia and has antibacterial potential. Purpose: To study the antibacterial potential of Butterfly Pea against Aa bacteria. Methods: Aa bacteria is cultured and the exposed to variations of Butterfly Pea extracts with various concentration such as 50%, 25%, 12.5%, 6.25%, and 3.125%. Positive control group is exposed to chlorhexidine and negative control group is exposed to DMSO. The results of diffusion test and dilution test are observed. Results: In the dilution test Aa bacteria grows 100% on negative control group, 73,32% on the extracts with 3.125% concentration, 29.32% on 6.25% concentration, 7.62% on 12.5% concentration, and 0% on 25% & 50% concentration and on positive control group. In the Aa bacterial diffusion test there was an inhibition in the positive group of 24.28 mm, the extract group 12.5%, 25%, and 50% could inhibit 10.45 mm, 13.42 mm, 15.61 mm and the extract group 6.25%, 3.125%, and negative control had no inhibition on bacteria. Conclusion: Potential Butterfly Pea flower extract as antibacteral compound against Aa with minimum inhibitory concentration of 12.5% and minimum bactericidal concentration of 25%. Butterfly pea flower extract (Clitoria ternatea) can inhibit Aggregatibacter actinomycetemcomitans bacteria with an average minimum inhibition zone diameter of 10.45 mm.