Experimental evidence on the impact of climate-induced hydrological and thermal variations on glacier-fed stream biofilms

Abstract

Climate change is predicted to alter the hydrological and thermal regimes of high-mountain streams, particularly glacier-fed streams. However, relatively little is known about how these environmental changes impact the microbial communities in glacier-fed streams. Here, we operated streamside flume mesocosms in the Swiss Alps, where benthic biofilms were grown under treatments simulating climate change. Treatments comprised four flow (natural, intermittent, stochastic, and constant) and two temperature (ambient streamwater and warming of +2°C) regimes. We monitored microbial biomass, diversity, community composition, and metabolic diversity in biofilms over 3 months. We found that community composition was largely influenced by successional dynamics independent of the treatments. While stochastic and constant flow regimes did not significantly affect community composition, droughts altered their composition in the intermittent regime, favouring drought-adapted bacteria and decreasing algal biomass. Concomitantly, warming decreased algal biomass and the abundance of some typical glacier-fed stream bacteria and eukaryotes, and stimulated heterotrophic metabolism overall. Our study provides experimental evidence towards potential and hitherto poorly considered impacts of climate change on benthic biofilms in glacier-fed streams.