Owing to its fast and reliable assessment of parasite growth, the SYBR® Green I-based fluorescence assay is widely used to monitor drug susceptibility of malaria parasites. Its particular advantages are that it is a simple, one-step procedure and very cost-effective making it especially suited for high through put screening of newly developed drugs and drug combinations. Here we describe a SYBR® Green I-based fluorescence assay protocol to be used for routine screening of compounds and extracts in a research laboratory environment. A variation of the standard protocol is also provided allowing to address stage-specific effects of fast-acting drugs.
CITATION STYLE
Leidenberger, M., Voigtländer, C., Simon, N., & Kappes, B. (2017). SYBR® green I-based fluorescence assay to assess cell viability of malaria parasites for routine use in compound screening. In Methods in Molecular Biology (Vol. 1601, pp. 97–110). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6960-9_9
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