Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

Abstract

Aim: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. Methods: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. Results: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%) compared to the control group (3.12±0.13%, P < 0.01). Conclusion: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro. © 2005 The WJG Press and Elsevier Inc. All rights reserved.