Relaxation of sheep cerebral arteries by vasoactive intestinal polypeptide and neurogenic stimulation: Inhibition by L-N(G)-monomethyl arginine in endothelium-denuded vessels

Abstract

1. Perivascular nerves of the sheep middle cerebral artery show immunoreactivity for both vasoactive intestinal polypeptide (VIP) and calcitonin gene-related peptide (CGRP). 2. Rings of endothelium-denuded sheep middle cerebral artery precontracted with 5-hydroxytryptamine were relaxed by CGRP (maximum relaxation = 87.8 ± 8.1%, pD2 = 7.81 ± 0.12, n = 12) and by VIP (maximum relaxation = 55.1 ± 4.1%, pD2 = 7.65 ± 0.04, n = 18). Rings of endothelium-denuded cat middle cerebral artery precontracted with U46619 were also relaxed by vasoactive intestinal polypeptide (maximum relaxation = 5.31 ± 6.1%, pD2 = 7.82 ± 0.11, n = 6). 3. Haemolysate (1 μl ml-1) inhibited VIP-induced relaxation in endothelium-denuded sheep and cat middle cerebral artery (n = 6) but had no effect on the CGRP-induced relaxation of the sheep middle cerebral artery (n = 6). 4. The relaxant response to VIP in endothelium-denuded sheep middle cerebral artery was inhibited by methylene blue (10 μM) and augmented by either M and B 22948 (10 μM) or superoxide dismutase (150 units ml-1). Indomethacin (1 μM) had no effect. 5. The addition of L-N(G)-monomethyl arginine (100 μM) inhibited both neurogenic and VIP-induced relaxation of endothelium-denuded sheep MCA by 56 ± 6% and 60 ± 6% (n = 5) respectively. The CGRP-induced relaxation was unaffected. 6. It is concluded that neurally mediated vasodilatation in the sheep middle cerebral artery is mediated largely by VIP through a direct action on smooth muscle through a cyclic-GMP-mediated mechanism that appears to involve synthesis of nitric oxide from L-arginine. Vasodilatation by CGRP, which is also contained in perivascular nerves, does not utilize this pathway.