A novel mechanism of pH buffering in c. Elegans glia: Bicarbonate transport via the voltage-gated C1C C1 channel CLH-1

19Citations
Citations of this article
39Readers
Mendeley users who have this article in their library.

Abstract

An important function of glia is the maintenance of the ionic composition and pH of the synaptic microenvironment. In terms of pH regulation, HCO3– buffering has been shown to be important in both glia and neurons. Here, we used in vivo fluorescent pH imaging and RNA sequencing of the amphid sheath glia of Caenorhabditis elegans to reveal a novel mechanism of cellular HCO3– uptake. While the classical mechanism of HCO3– uptake involves Na+/HCO3– cotransporters, here we demonstrate that the C. elegans ClC Cl– channel CLH-1 is highly permeable to HCO3– and mediates HCO3 uptake into amphid sheath glia. CLH-1 has homology and electrophysiological properties similar to the mammalian ClC-2 Cl– channel. Our data suggest that, in addition to maintaining synaptic Cl concentration, these channels may also be involved in maintenance of synaptic pH via HCO3– flux. These findings provide an exciting new facet of study regarding how pH is regulated in the brain.

Cite

CITATION STYLE

APA

Grant, J., Matthewman, C., & Bianchi, L. (2015). A novel mechanism of pH buffering in c. Elegans glia: Bicarbonate transport via the voltage-gated C1C C1 channel CLH-1. Journal of Neuroscience, 35(50), 16377–16397. https://doi.org/10.1523/JNEUROSCI.3237-15.2015

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free