New Exo-type Arabinase from Erwinia carotovora IAM 1024

Abstract

An arabinase has been purified from the culture fluid of Erwinia carotovora IAM 1024 grown on a medium containing arabinan. The purified enzyme was active on beet arabinan and inactive on 1,5-arabinan, arabinoxylan, arabinogalactan, and p-nitrophenyl α-L-arabinofuranoside. The optimum pH was 6.0. The enzyme was stable over a pH range of 5.0 to 11.0. When the purified enzyme acted on beet arabinan, the main product was arabinotriose. © 1984, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.