RNA extraction resulting in good yields and quality is a fundamental step for the analyses of transcriptomes through high-throughput sequencing technologies, microarray, and also northern blots, RT-PCR, and RTqPCR. Even though many specific protocols designed for plants with high content of secondary metabolites have been developed, these are often expensive, time consuming, and not suitable for a wide range of tissues. Here we present a modification of the method previously described using the commercially available Concert™ Plant RNA Reagent (Invitrogen) buffer for field-grown adult pine trees with high oleoresin content.
CITATION STYLE
de Lima, J. C., Füller, T. N., de Costa, F., Rodrigues-Corrêa, K. C. S., & Fett-Neto, A. G. (2016). A modified protocol for high-quality RNA extraction from oleoresin-producing adult pines. In Methods in Molecular Biology (Vol. 1405, pp. 27–33). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3393-8_3
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