The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.
CITATION STYLE
Milberg, O., Tora, M., Shitara, A., Takuma, T., Masedunskas, A., & Weigert, R. (2014). Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy. Methods in Molecular Biology, 1174, 407–421. https://doi.org/10.1007/978-1-4939-0944-5_28
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