Pullulan Production from Lignocellulosic Plant Biomass or Starch-Containing Processing Coproduct Hydrolysates

Abstract

The complex polysaccharide pullulan is characterized as a glucose-containing biopolymer that is both water-soluble and neutral in polarity. A variety of commercial applications exist for pullulan, including its utilization as a flocculant, a blood plasma substitute, a food additive, a dielectric material, an adhesive, or a packaging film. The fungus Aureobasidium pullulans has used several hydrolysates derived from plant biomass or starch-containing processing coproducts to support polysaccharide production. These include various plant biomass or processing coproduct streams such as lignocellulosic-containing peat, prairie grass, stalks, hulls, straw, shells, and pods or starch-containing coproducts from the processing of corn, rice, jackfruit seeds, palm kernels, cassava, and potatoes. The pullulan concentration produced by A. pullulans and the pullulan content of the polysaccharide depend on the plant hydrolysate carbon content and the strain used. If a lower-cost culture medium for fungal pullulan production were to be developed, a more economical approach to synthesizing commercial pullulan would be the utilization of plant-derived hydrolysates. This review examines the ability of selected hydrolysates of lignocellulosic plant biomass or plant-derived starch-containing processing coproducts to support A. pullulans polysaccharide synthesis in order to identify those substrates with the greatest potential for reducing the cost of commercial pullulan.