Activation of the cAMP pathway induces RACK1-dependent binding of β-actin to BDNF promoter

5Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

Abstract

RACK1 is a scaffolding protein that contributes to the specificity and propagation of several signaling cascades including the cAMP pathway. As such, RACK1 participates in numerous cellular functions ranging from cell migration and morphology to gene transcription. To obtain further insights on the mechanisms whereby RACK1 regulates cAMP-dependent processes, we set out to identify new binding partners of RACK1 during activation of the cAMP signaling using a proteomics strategy. We identified β-actin as a direct RACK1 binding partner and found that the association between β-actin and RACK1 is increased in response to the activation of the cAMP pathway. Furthermore, we show that cAMP-dependent increase in BDNF expression requires filamentous actin. We further report that β-actin associates with the BDNF promoter IV upon the activation of the cAMP pathway and present data to suggest that the association of β-actin with BDNF promoter IV is RACK1-dependent. Taken together, our data suggest that β-actin is a new RACK1 binding partner and that the RACK1 and β-actin association participate in the cAMP-dependent regulation of BDNF transcription.

Cite

CITATION STYLE

APA

Neasta, J., Fiorenza, A., He, D. Y., Phamluong, K., Kiely, P. A., & Ron, D. (2016). Activation of the cAMP pathway induces RACK1-dependent binding of β-actin to BDNF promoter. PLoS ONE, 11(8). https://doi.org/10.1371/journal.pone.0160948

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free