Activity-dependent IGF-1 exocytosis is controlled by the Ca 2+-sensor synaptotagmin-10

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Abstract

Synaptotagmins Syt1, Syt2, Syt7, and Syt9 act as Ca2+-sensors for synaptic and neuroendocrine exocytosis, but the function of other synaptotagmins remains unknown. Here, we show that olfactory bulb neurons secrete IGF-1 by an activity-dependent pathway of exocytosis, and that Syt10 functions as the Ca2+-sensor that triggers IGF-1 exocytosis in these neurons. Deletion of Syt10 impaired activity-dependent IGF-1 secretion in olfactory bulb neurons, resulting in smaller neurons and an overall decrease in synapse numbers. Exogenous IGF-1 completely reversed the Syt10 knockout phenotype. Syt10 colocalized with IGF-1 in somatodendritic vesicles of olfactory bulb neurons, and Ca2+-binding to Syt10 caused these vesicles to undergo exocytosis, thereby secreting IGF-1. Thus, Syt10 controls a previously unrecognized pathway of Ca2+-dependent exocytosis that is spatially and temporally distinct from Ca2+-dependent synaptic vesicle exocytosis controlled by Syt1. Our findings thereby reveal that two different synaptotagmins can regulate functionally distinct Ca2+-dependent membrane fusion reactions in the same neuron. © 2011 Elsevier Inc.

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Cao, P., Maximov, A., & Südhof, T. C. (2011). Activity-dependent IGF-1 exocytosis is controlled by the Ca 2+-sensor synaptotagmin-10. Cell, 145(2), 300–311. https://doi.org/10.1016/j.cell.2011.03.034

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