We have studied the acyl-chain conformation in stalk phases of model membranes by x-ray diffraction from oriented samples. As an equilibrium lipid phase induced by dehydration, the stalk or rhombohedral phase exhibits lipidic passages (stalks) between adjacent bilayers, representing a presumed intermediate state in membrane fusion. From the detailed analysis of the acyl-chain correlation peak, we deduce the structural parameters of the acyl-chain fluid above, at, and below the transition from the lamellar to rhombohedral state, at the molecular level. © 2012 Biophysical Society.
Weinhausen, B., Aeffner, S., Reusch, T., & Salditt, T. (2012). Acyl-chain correlation in membrane fusion intermediates: X-ray diffraction from the rhombohedral lipid phase. Biophysical Journal, 102(9), 2121–2129. https://doi.org/10.1016/j.bpj.2012.03.069