Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor

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Abstract

The in vitro oxidation of the two polycyclic aromatic hydrocarbons anthracene and benzo[a]pyrene, which have ionization potentials of ≤7.45 eV, is catalyzed by laccases from Trametes versicolor. Crude laccase preparations were able to oxidize both anthracene and the potent carcinogen benzo[a]pyrene. Oxidation of benzo[a]pyrene was enhanced by the addition of the cooxidant 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), while an increased anthracene oxidizing ability was observed in the presence of the low-molecular-weight culture fluid ultrafiltrate. Two purified laccase isozymes from T. versicolor were found to have similar oxidative activities towards anthracene and benzo[a]pyrene. Oxidation of anthracene by the purified isozymes was enhanced in the presence of ABTS, while ABTS was essential for the oxidation of benzo[a]pyrene. In all cases anthraquinone was identified as the major end product of anthracene oxidation. These findings indicate that laccases may have a role in the oxidation of polycyclic aromatic hydrocarbons by white rut fungi.

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Collins, P. J., Kotterman, M. J. J., Field, J. A., & Dobson, A. D. W. (1996). Oxidation of anthracene and benzo[a]pyrene by laccases from Trametes versicolor. Applied and Environmental Microbiology, 62(12), 4563–4567. https://doi.org/10.1128/aem.62.12.4563-4567.1996

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