Utilization of various encapsulation methods in proteolytic bacteria to maintain biomass cells during storage

Abstract

Bacterial isolates used are proteolytic bacteria. Bacterial cell encapsulation aimed to protect cells from some external environmental factors that can interfere with the metabolism of the cell, so as to maintain the viability of bacteria, extending the shelf life and facilitate the distribution and utilization. In this study, the encapsulation of the bacteria was carried out by nanoencapsulation method using a spray dyer and electrospun nanofibers. Encapsulation in nanosize has advantages such as immobilization efficiency because of the increased surface area and volume, providing protection due to the increased flexibility of the encapsulation material. Nanoencapsulation the proteolytic bacteria was using the coating material in the form of skim milk and maltodextrin. Electrospun nanofiber for proteolytic bacteria was using polyvinyl alcohol (PVA) and skim milk as a coating material. The results of the viability of proteolytic nanoencapsulation were higher when encapsulated using skim milk at a concentration of 15% was 44.07%. Results of encapsulation of bacteria using electrospun nanofiber method showed three parameters that affected the process of electrospun nanofiber: the coating material parameters (concentration, viscosity and mixing ratio), the process parameters (voltage, flow rate, and the distance between the collector and needle), and environmental parameters (relative humidity and temperature).