Biological synthesis of AgNPs BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING THE TUBEROUS ROOT EXTRACT OF IPOMOEA BATATAS AND THEIR CHARACTERIZATIONS AND ANTIBACTERIAL ACTIVITY

Abstract

Objectives: Tuberous root extract based synthesis of silver nanoparticles (AgNPs), characterizations using Fourier-transform infrared spectroscopy (FT-IR), UV-visible, powder X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) techniques, and antibacterial activity of AgNPs against Staphylococcus aureus, Staphylococcus mutans, Proteus vulgaris, and Escherichia coli. Methods: Root extract of Ipomoea batatas (sweet potato) was prepared by boiling of small cut pieces of root with double distilled water. Added root extract with silver nitrate solution and centrifuged and collect the pellets. After several washing and drying, AgNPs have been preserved for characterizations and antibacterial activity. Results: The synthesized AgNPs were well characterized by FT-IR, UV-visible, XRD, FESEM, and EDX methods, and significant zones of inhibition observed around the loaded AgNPs on the agar plates. The zones of inhibition have been achieved 36, 40, 46, and 32 mm for E. coli, P. vulgaris, S. mutans, and S. aureus. Conclusion: The characterisation methods such as UV-Visible, FTIR, Powder XRD, FESEM and EDX indicate an efficient formation of AgNPs using root extract of I. batatas. The biologically synthesized AgNPs are found good antibacterial agents.