MiR-146a-5p suppresses ATP-binding cassette subfamily G member 1 dysregulation in patients with refractory Mycoplasma pneumoniae via interleukin 1 receptor-associated kinase 1 downregulation

Abstract

In the present study, we examined the function of microRNA (miR)-146a-5p in patients with refractory Mycoplasma pneumoniae pneumonia. In brief, the expression of miR-146a-5p was reduced in patients with refractory Mycoplasma pneumoniae pneumonia. Downregulation of miR-146a-5p reduced inflammation in an in vitro model of refractory Mycoplasma pneumoniae pneumonia, whilst overexpression of miR-146a-5p promoted inflammation. Downregulation of miR-146a-5p induced the protein expression of ATP-binding cassette subfamily G member 1 (ABCG1) and interleukin 1 receptor-associated kinase 1 (IRAK-1), while suppressed expression was observed of the aforementioned proteins following overexpression of miR-146a-5p in an in vitro model of refractory Mycoplasma pneumoniae pneumonia. The administration of small interfering RNA against RXR or IRAK-1 attenuated the effects of miR-146a-5p on inflammation in an in vitro model of refractory Mycoplasma pneumoniae pneumonia. Collectively, these results suggested that miR-146a-5p reduced ABCG1 expression in refractory Mycoplasma pneumoniae pneumonia via downregulation of IRAK-1.