MicroRNA‑26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer

Abstract

MicroRNAs (miRNAs/miRs) play key roles in cancer progression. Extensive research has revealed that miR‑26a is abnormally expressed and functions as a tumor suppressor in numerous types of cancer. Thus, the present study was undertaken to investigate the regulatory role and potential mechanism of action of miR‑26a in breast cancer. Furthermore, the present study aimed to examine the altera‑ tions in miR‑26a expression and its effects on human breast cancer cells. Reverse transcription‑quantitative PCR was conducted to assess the differences in miR‑26a expression between human breast cancer and normal breast specimens. A Cell Counting Kit‑8 assay and cloning experiments were used to detect cell proliferation and clone formation. Wound healing and Transwell assays were performed to examine cell migration and invasion. A luciferase activity experiment was utilized to validate the association between miR‑26a and family with sequence similarity 98 member A (FAM98A). Western blotting was conducted to detect the protein expres‑ sion levels of FAM98A, sonic hedgehog signaling molecule (SHH), smoothened, frizzled class receptor (SMO) and GLI family zinc finger 1 (GLI1). The results indicated that miR‑26a expression was decreased in breast carcinoma tissues and