Analysis of phenolic compounds and antioxidant activity in wild blackberry fruits

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© 2015 by the authors; licensee MDPI, Basel, Switzerland. Twenty three different wild blackberry fruit samples were assessed regarding their phenolic profiles and contents (by LC/MS quadrupole time-of-flight (QTOF) and antioxidant activity (ferric reducing ability of plasma (FRAP) and 2,2-azinobis (3-ethyl-benzothiazoline- 6-sulfonic acid) (ABTS)) by two different extraction methods. Thirty four phenolic compounds were detected (8 anthocyanins, 15 flavonols, 3 hydroxycinnamic acids, 6 ellagic acid derivatives and 2 flavones). In samples, where pressurized liquid extraction (PLE) was used for extraction, a greater increase in yields of phenolic compounds was observed, especially in ellagic acid derivatives (max. 59%), flavonols (max. 44%) and anthocyanins (max. 29%), than after extraction by the ultrasonic technique extraction (UAE) method. The content of phenolic compounds was significantly correlated with the antioxidant activity of the analyzed samples. Principal component analysis (PCA) revealed that the PLE method was more suitable for the quantitative extraction of flavonols, while the UAE method was for hydroxycinnamic acids.




Oszmiański, J., Nowicka, P., Teleszko, M., Wojdyło, A., Cebulak, T., & Oklejewicz, K. (2015). Analysis of phenolic compounds and antioxidant activity in wild blackberry fruits. International Journal of Molecular Sciences, 16(7), 14540–14553.

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