Analysis of the promoter region of the gene LIP1 encoding triglyceride lipase from Fusarium graminearum

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Abstract

Triglyceride lipases catalyze the reversible degradation of glycerol esters with long-chain fatty acids into fatty acids and glycerol. In silico analysis of 5'-end flanking sequence of the gene LIP1 encoding a triglyceride lipase from the wheat head blight pathogen Fusarium graminearum revealed the presence of several cis-regulatory elements. To delineate the function of these regulatory elements, we constructed a series of deletion mutants in the LIP1 promoter region fused to the open reading frame of a green fluorescent protein (GFP) and assayed the promoter activity. Analysis of GFP expression levels in mutants indicated that a 563-bp promoter sequence was sufficient to drive the expression of LIP1 and regulatory elements responsible for the gene induction were located within the 563-372. bp region. To further investigate the regulatory elements, putative cis-acting elements spanned within the 563-372. bp region were mutated using a targeted mutagenesis approach. A CCAAT box, a CreA binding site, and a fatty acid responsive element (FARE) were identified and confirmed to be required for the basal expression of LIP1, glucose suppression and fatty acid induction, respectively. © 2011 Elsevier GmbH.

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APA

Feng, J., Bhadauria, V., Liu, G., Selvaraj, G., Hughes, G. R., & Wei, Y. (2011). Analysis of the promoter region of the gene LIP1 encoding triglyceride lipase from Fusarium graminearum. Microbiological Research, 166(8), 618–628. https://doi.org/10.1016/j.micres.2010.12.002

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