Anion channel inhibitor NPPB-inhibited fluoride accumulation in tea plant (Camellia sinensis) is related to the regulation of Ca2+, CaM and depolarization of plasma membrane potential

6Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

© 2016 by the authors; licensee MDPI, Basel, Switzerland. Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca 2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca 2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca 2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca 2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca 2+ and H + efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca 2+ -CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants.

Cite

CITATION STYLE

APA

Zhang, X. C., Gao, H. J., Yang, T. Y., Wu, H. H., Wang, Y. M., Zhang, Z. Z., & Wan, X. C. (2016). Anion channel inhibitor NPPB-inhibited fluoride accumulation in tea plant (Camellia sinensis) is related to the regulation of Ca2+, CaM and depolarization of plasma membrane potential. International Journal of Molecular Sciences, 17(1), 2–14. https://doi.org/10.3390/ijms17010057

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free